Expression profiles of growth hormone receptor and insulin-like growth factor I in cattle and yak tissues revealed by quantitative real-time PCR

. Pei, J.; Lang, X.; Bao, P.; Liang, C.; Chu, M.; Feng, R.; Yan, P.

The goals of this study were to compare the mRNA expression profiles of growth hormone recep tor (GHR) and insulin-like growth factor I (IGF-I) in various tissues of cattle and the semi-wild yak (Datong yak) and to find out whether the mRNA levels of the two genes are correlated. The mRNA levels of GHR and IGF-I in heart, lung, liver, spleen, pancreas, kidney, muscle, mammary gland and ovary of cattle and yak were investigated by using quantitative real-time po ly mer ase chain reaction (PCR). The experiments showed that the transcript levels of the two genes were signif icantly higher in liver ( P<0.05) than in the other tissues for both species and that IGF-I levels varied more among tissues ( P<0.01) than did GHR levels. The GHR tran script level in pancreas was higher in yak ( P<0.05) than in cattle. There was no statistically sig nif i cant difference in IGF-I tran script levels among all the tissues of both bovine groups. Growth hormone receptor and IGF-I transcript levels were positively correlated in mammary gland ( P<0.01), lung ( P<0.05) and muscle ( P<0.05) in yak, negatively correlated in cattle heart ( P<0.05) and not correlated in the other tissues. The results indicate that the two genes are reg u lated differently in various tissues under normal physiological conditions in these two bovine species.

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. Pei, J. / Lang, X. / Bao, P. / et al: Expression profiles of growth hormone receptor and insulin-like growth factor I in cattle and yak tissues revealed by quantitative real-time PCR. 2013. FBN Dummerstorf.

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