Sister chromatid exchange analysis in cats ( Felis catus)

Szeleszczuk, Olga; Kuchta-Gładysz, Marta; Wójcik, Ewa; Otwinowska-Mindur, Agnieszka; Wojnar, Tomasz; Godynia, Beata; Niedbała, Piotr

Sister chromatid exchange (SCE) is one of the cytogenetic methods which diagnoses damage to chromosomes and allows evaluation of the mutagenic influence of a given factor on a cell’s DNA. The purpose of the experiment was to determine the level of spontaneous and inductive SCE in the domestic cat. The research was carried out on 23 domestic cats Felis catus. Chromosome preparations were prepared from lymphocytes of peripheral blood after 72 h of in vitro breeding with the addition of bromodeoxyuridine (BrdU) in five different concentrations: 0.25, 0.5, 1.0, 2.5, 5.0 μg/ml. Chromosomes were stained by means of the fluorescence plus Giemsa (FPG) technique in order to carry out microscopic analysis. It was stated that the level of spontaneous SCE in the domestic cat occurs at a concentration 0.5 μg/ml on the basis of research previously carried out. Higher concentrations of this substance have a genotoxic action and damage DNA of chromosomes and induct additional SCEs in chromosomes of this species. Moreover, it was stated that the number of SCEs is higher in males than females. Our research also proved that the number of exchanges increases along with age in cats of both sexes.

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Szeleszczuk, Olga / Kuchta-Gładysz, Marta / Wójcik, Ewa / et al: Sister chromatid exchange analysis in cats (Felis catus). 2014. FBN Dummerstorf.

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