Li, Yang; Wu, Zhaojun; Dong, Xingchen; Xu, Zifu; Zhang, Qixin; Su, Haiyan; Jia, Zhongjun; Sun, Qingye

Polymetallic mine tailings have great potential as carbon sequestration tools to stabilize atmospheric inline-formulaCO2 concentrations. However, previous studies focused on carbonate mineral precipitation, whereas the role of autotrophic bacteria in mine tailing carbon sequestration has been neglected. In this study, carbon sequestration in two samples of mine tailings treated with inline-formulaFeS2 was evaluated using inline-formula13C isotope, pyrosequencing and DNA-based stable isotope probing (SIP) analyses to identify carbon fixers. Mine tailings treated with inline-formulaFeS2 exhibited a higher percentage of inline-formula13C atoms (inline-formula1.76±0.06 % for Yangshanchong and inline-formula1.36±0.01 % for Shuimuchong) than did controls over a 14-day incubation, which emphasized the role of autotrophs in carbon sequestration with pyrite addition. Pyrite treatment also led to changes in the composition of bacterial communities, and several autotrophic bacteria increased, including Acidithiobacillus and Sulfobacillus. Furthermore, pyrite addition increased the relative abundance of the dominant genus Sulfobacillus by 8.86 % and 5.99 % in Yangshanchong and Shuimuchong samples, respectively. Furthermore, DNA SIP results indicated a 8.20–16.50 times greater gene copy number for cbbL than cbbM in inline-formula13C-labeled heavy fractions, and a Sulfobacillus-like cbbL gene sequence (cbbL-OTU1) accounted for 30.11 %–34.74 % of all cbbL gene sequences in inline-formula13C-labeled heavy fractions of mine tailings treated with inline-formulaFeS2. These findings highlight the importance of the cbbL gene in bacterial carbon sequestration and demonstrate the ability of chemoautotrophs to sequester carbon during sulfide mineral oxidation in mine tailings. This study is the first to investigate carbon sequestration by autotrophic bacteria in mine tailings through the use of isotope tracers and DNA SIP.



Li, Yang / Wu, Zhaojun / Dong, Xingchen / et al: cop_mods_00003290. 2019. Copernicus Publications.


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