Variation in the caprine keratin-associated protein 15-1 (KAP15-1) gene affects cashmere fibre diameter

Zhao, Mengli; Zhou, Huitong; Hickford, Jon G. H.; Gong, Hua; Wang, Jiqing; Hu, Jiang; Liu, Xiu; Li, Shaobin; Hao, Zhiyun; Luo, Yuzhu

Keratin-associated proteins (KAPs) are a structural component of cashmere fibre, and variation in some KAP genes (KRTAPs) has been associated with a number of caprine fibre traits. In this study, we report the identification of KRTAP15-1 in goats. Sequence variation in the gene was detected using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) technique in 250 Longdong goats, and six variants (named A to F) containing eight single nucleotide polymorphisms (SNPs) were identified. Five of the SNPs were non-synonymous and would lead to putative amino acid changes. Reverse-transcription polymerase chain reaction (RT-PCR) analysis revealed that KRTAP15-1 was expressed in secondary hair follicles but not in heart tissue, liver tissue, lung tissue, kidney tissue or the longissimus dorsi muscle. Despite being rich in cysteine, the caprine KAP15-1 protein possesses a high content of serine and moderate content of glycine and phenylalanine. Association analyses revealed that KRTAP15-1 variant A was associated with decreased mean fibre diameter (MFD), and this effect appeared to be dominant; while variant C was found to be associated with increased MFD, the effect being recessive. The findings suggest that caprine KRTAP15-1 is highly polymorphic and that variation in this gene affects cashmere MFD.



Zhao, Mengli / Zhou, Huitong / Hickford, Jon G. H. / et al: Variation in the caprine keratin-associated protein 15-1 (KAP15-1) gene affects cashmere fibre diameter. 2019. Copernicus Publications.


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