In vivo monitoring of hepatic glycolipid distribution of n-6 ∕  n-3 in jugular-vein-cannulated rats as a nutritional research model for monogastric animal

Park, Sang-O.; Zammit, Victor A.

The metabolic distribution via blood from liver of glycerolipids by omega-6 to omega-3 fatty acid (inline-formulan-6 inline-formula∕inline-formulan-3) ratio in monogastric animal nutrition is very important. In vivo monitoring technique using jugular-vein-cannulated rats as a nutritional model for monogastric animal can yield important insights into animal nutrition. This study was conducted to determine the effect of different inline-formulan-6 inline-formula∕inline-formulan-3 ratios (inline-formula71:1, inline-formula4:1, inline-formula15:1, inline-formula30:1) on metabolic distribution of glycerolipids newly synthesized and secreted in the liver of the rats and explore the mechanism involved. Regarding inline-formula14CO2 released from oxidation of glycerolipid metabolism, it was the highest (22.5 %) in groups with a inline-formulan-6 inline-formula∕inline-formulan-3 ratio of inline-formula4:1 (inline-formulaP<0.05). The control group showed the highest total glycerolipid level, followed by the inline-formula30:1, inline-formula15:1, and inline-formula4:1 groups in order (inline-formulaP<0.05). When secreted triacylglycerol level of each group was compared with that of the control group, the inline-formula4:1, inline-formula15:1, and inline-formula30:1 groups were decreased by 36.3 %, 20.9 %, and 13.3 %, respectively (inline-formulaP<0.05). Regarding the distribution of phospholipid against total glycerolipid compared to the control group, the inline-formula4:1, inline-formula15:1, and inline-formula30:1 groups were 1.38, 1.29, and 1.17 times higher, respectively (inline-formulaP<0.05). In the comparison of inline-formula14CO2 emission against total glycerolipid compared with the control group, the inline-formula4:1, inline-formula15:1, and inline-formula30:1 groups were 1.61, 1.52, and 1.29 times higher, respectively (inline-formulaP<0.05). These results demonstrate that a dietary inline-formulan-6 inline-formula∕inline-formulan-3 fatty acid ratio of inline-formula4:1 could significantly decrease harmful lipid levels in the blood by controlling the mechanism of metabolic distribution via blood from triglyceride and phospholipid newly synthesized in the liver of cannulated rat.

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Zitierform:

Park, Sang-O. / Zammit, Victor A.: In vivo monitoring of hepatic glycolipid distribution of n-6 ∕ n-3 in jugular-vein-cannulated rats as a nutritional research model for monogastric animal. 2019. Copernicus Publications.

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